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Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10 ArchiMer
Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto.
A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its...
Tipo: Text Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00171/28260/26530.pdf
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Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10 ArchiMer
Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto.
A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its...
Tipo: Text Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00372/48356/48545.pdf
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New bioemulsifiers produced by Candida lipolytica using D-glucose and babassu oil as carbon sources BJM
Vance-Harrop,Mabel H.; Gusmão,Norma B. de; Campos-Takaki,Galba Maria de.
Candida lipolytica IA 1055 produced extracellular biosurfactants with emulsification activity by fermentation using babassu oil and D-glucose as carbon sources. Natural seawater diluted at 50% supplemented with urea, ammonium sulfate, and phosphate was used as economic basal medium. The best results were achieved with the YSW-B2 medium, which contained urea, ammonium sulfate, and babassu oil and with YSW-B3 medium, which contained urea, ammonium sulfate, phosphate, and babassu oil, kept under fed batch fermentation for 60 hours with 5% of babassu oil. For the two media, the maximum specific growth rates were 0.02 h-1 and 0.04 h-1; the generation times were 34.6 h-1 and 17.3 h-1, and the emulsification activities were 0.666 and 0.158 units, respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioemulsifier; Candida lipolytica; Seawater; Babassu oil.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200006
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Obtenção e caracterização de manoproteínas da parede celular de leveduras de descarte em cervejaria - doi: 10.4025/actascibiolsci.v34i1.7124 Biological Sciences
Costa, Ariane Gaspar; Universidade Estadual de Londrina - UEL; Magnani, Marciane; Universidade Estadual de Londrina - UEL; Castro-Gomez, Raul Jorge Hernan; Universidade Estadual de Londrina - UEL.
A biomass de levedura resultante da produção de cerveja é mátéria-prima para extração de componentes celulares, incluíndo manoproteínas. O presente trabalho avaliou a possibilidade da utilização da levedura Saccharomyces sp. descartada em cervejaria, para obtenção de extrato com manoproteínas. A extração foi conduzida segundo delineamento fatorial incompleto, Box-Behnken 33, para as variáveis temperaturas (75, 85 e 95ºC), tempo de extração (5, 7 e 9h) e concentração da suspensão de parede celular (10, 15 e 20%). O etanol residual da fermentação não interfere na obtenção do extrato contendo manoproteínas. O maior índice de extração foi 4,08%, observado para temperatura de 95ºC na concentração de 10% por 7h e 15% por 9h. A validação experimental do maior...
Tipo: Composição bioquímica Palavras-chave: 212.00.00.09 - Microbiologia Saccharomyces sp.; Bioemulsificante; Levedura cervejeira 2.08.02.00-5 - Bioquímica dos Microorganismos Saccharomyces sp.; Bioemulsifier; Brewery yeast.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7124
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Production and characterization of surface-active compounds from Gordonia amicalis BABT
Jackisch-Matsuura,Ani Beatriz; Santos,Leonardo Silva; Eberlin,Marcos Nogueira; Faria,Andréia Fonseca de; Matsuura,Takeshi; Grossman,Matthew James; Durrant,Lucia Regina.
Two methods were used to make crude preparations of surface-active compounds (SACs) produced by Gordonia amicalis grown on the medium containing 1% diesel oil. Using a 2:1 (v/v) solution of chloroform:methanol for extraction, Type I SACs were isolated and shown to produce oil in water (O/W) emulsions. Type II SACs were isolated by precipitation with ammonium sulfate and produced predominantly water in oil emulsions (W/O). The crude Type I and II preparations were able to produce a significant reduction in the surface tension of water; however, the crude Type II preparation had 10-25 fold higher emulsification activity than the Type I preparation. Both SAC preparations were analyzed by the TLC and each produced two distinct bands with Rf 0.44 and 0.62 and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioemulsifier; Bioremediation; Biosurfactant; Gordonia amicalis; Industrial chemicals; Microbial surfactant production.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100019
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